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Promega e. coli bl21 (krx #l3002)
E. Coli Bl21 (Krx #L3002), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e%2E+coli+bl21+(krx/e++coli+bl21+de3+plyss/pm36614024-635-6-11
Average 90 stars, based on 1 article reviews
e. coli bl21 (krx #l3002) - by Bioz Stars, 2026-10
90/100 stars

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Article Title: α N -Acetyl β-Endorphin Is an Endogenous Ligand of σ1Rs That Regulates Mu-Opioid Receptor Signaling by Exchanging G Proteins for σ2Rs in σ1R Oligomers.
Article Snippet: The vector was introduced into the E. coli BL21 (KRX #L3002, Promega) and clones were selected on solid medium containing ampicillin.

Article Title: Calmodulin Supports TRPA1 Channel Association with Opioid Receptors and Glutamate NMDA Receptors in the Nervous Tissue
Article Snippet: The vectors were introduced into E. coli BL21 (KRX #L3002, Promega) and clones were selected on solid medium containing ampicillin.

Article Title: The σ1 Receptor and the HINT1 Protein Control α2δ1 Binding to Glutamate NMDA Receptors: Implications in Neuropathic Pain
Article Snippet: The vector was introduced into the E. coli BL21 (KRX #L3002, Promega, Madison, WI, USA) and clones were selected on solid medium containing ampicillin.

Article Title: Human HINT1 Mutant Proteins that Cause Axonal Motor Neuropathy Exhibit Anomalous Interactions with Partner Proteins.
Article Snippet: The vector was introduced into the E. coli BL21 (KRX #L3002, Promega), and clones were selected on solid medium containing ampicillin.

Polymerase Chain Reaction:

Article Title: tRNA Arg binds in vitro TDP-43 RNA recognition motifs and ligand of Ate1 protein LIAT1
Article Snippet: Given that the partner proteins of LIAT1 are involved in different aspects of RNA metabolism, it is foreseeable that LIAT1 capacity to bind RNA could have functional implications which may reflect also on TDP-43 proteostasis. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C.

Construct:

Article Title: tRNA Arg binds in vitro TDP-43 RNA recognition motifs and ligand of Ate1 protein LIAT1
Article Snippet: Given that the partner proteins of LIAT1 are involved in different aspects of RNA metabolism, it is foreseeable that LIAT1 capacity to bind RNA could have functional implications which may reflect also on TDP-43 proteostasis. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C.

Clone Assay:

Article Title: tRNA Arg binds in vitro TDP-43 RNA recognition motifs and ligand of Ate1 protein LIAT1
Article Snippet: Given that the partner proteins of LIAT1 are involved in different aspects of RNA metabolism, it is foreseeable that LIAT1 capacity to bind RNA could have functional implications which may reflect also on TDP-43 proteostasis. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C.

Article Title: The sigma-1 receptor curtails endogenous opioid analgesia during sensitization of TRPV1 nociceptors.
Article Snippet: Department of Pharmacology, Faculty of Medicine, University of Granada, Granada, Spain Institute of Neuroscience, Biomedical Research Center, University of Granada, Granada, Spain Biosanitary Research Institute ibs.. GRANADA, Granada, Spain Department of Translational Neurosciences, Neuropharmacology, Cajal Institute, CSIC, Madrid, Spain Department of Neurobiology, Harvard Medical School, Boston, MA, USA F.M.. Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA Te ofilo Hernando Institute for Drug Discovery, Madrid, Spain

Article Title: The Axonal Motor Neuropathy-Related HINT1 Protein Is a Zinc- and Calmodulin-Regulated Cysteine SUMO Protease
Article Snippet: .. The vector was introduced into Escherichia coli BL21 (KRX #L3002; Promega), and clones were selected on solid medium containing ampicillin. ..

Article Title: The ALS-Related σ1R E102Q Mutant Eludes Ligand Control and Exhibits Anomalous Response to Calcium
Article Snippet: The PCR products were cloned downstream of the glutathione S-transferase (GST)/HaloTag coding sequence (Flexi Vector, Promega Biotech Iberica, Madrid, Spain) and the tobacco etch virus (TEV) protease site, and sequencing revealed that the sequences of the proteins were identical to the GenBank sequences. .. The vectors were introduced into Escherichia coli BL21 (KRX #L3002, Promega), and clones were selected on solid medium containing ampicillin. ..

Transformation Assay:

Article Title: tRNA Arg binds in vitro TDP-43 RNA recognition motifs and ligand of Ate1 protein LIAT1
Article Snippet: Given that the partner proteins of LIAT1 are involved in different aspects of RNA metabolism, it is foreseeable that LIAT1 capacity to bind RNA could have functional implications which may reflect also on TDP-43 proteostasis. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C.

Recombinant:

Article Title: tRNA Arg binds in vitro TDP-43 RNA recognition motifs and ligand of Ate1 protein LIAT1
Article Snippet: Given that the partner proteins of LIAT1 are involved in different aspects of RNA metabolism, it is foreseeable that LIAT1 capacity to bind RNA could have functional implications which may reflect also on TDP-43 proteostasis. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C.

Expressing:

Article Title: tRNA Arg binds in vitro TDP-43 RNA recognition motifs and ligand of Ate1 protein LIAT1
Article Snippet: Given that the partner proteins of LIAT1 are involved in different aspects of RNA metabolism, it is foreseeable that LIAT1 capacity to bind RNA could have functional implications which may reflect also on TDP-43 proteostasis. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C. .. Synthetic genes encoding Homo sapiens TDP-43 (UniprotKB entry Q13148) or Mus musculus LIAT1 (UniProtKB- Q810M6) were used for PCR production of DNA amplicons encoding full length multidomain constructs of the conserved region of human TDP-43 (hTDP-43) constructs and mouse LIAT1 (mLIAT1) which were then cloned in pET-LIC vectors 2B-T, 2Bc-T, 2T-T and 2TcT conferring an amino-terminal His 6 , carboxyl-terminal His 6 , amino terminal His 6 -thioredoxin, or carboxyl-terminal His 6 - thioredoxin tags (respectively), and a TEV cleavage site, transformed in Escherichia coli BL21 KRX cells (Promega) then grown in Terrific Broth medium supplemented with 100 μg L -1 ampicillin at 37°C until OD 600 ~ 0.6 when recombinant protein expression was induced with 1mM IPTG and 0.2 % rhamnose for 12 hr at 20°C.

Plasmid Preparation:

Article Title: The sigma-1 receptor curtails endogenous opioid analgesia during sensitization of TRPV1 nociceptors.
Article Snippet: Department of Pharmacology, Faculty of Medicine, University of Granada, Granada, Spain Institute of Neuroscience, Biomedical Research Center, University of Granada, Granada, Spain Biosanitary Research Institute ibs.. GRANADA, Granada, Spain Department of Translational Neurosciences, Neuropharmacology, Cajal Institute, CSIC, Madrid, Spain Department of Neurobiology, Harvard Medical School, Boston, MA, USA F.M.. Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA Te ofilo Hernando Institute for Drug Discovery, Madrid, Spain

Article Title: The Axonal Motor Neuropathy-Related HINT1 Protein Is a Zinc- and Calmodulin-Regulated Cysteine SUMO Protease
Article Snippet: .. The vector was introduced into Escherichia coli BL21 (KRX #L3002; Promega), and clones were selected on solid medium containing ampicillin. ..



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